1kg 1.5kg Sterilised Grain Spawn Bags WITH Injection Port, Sterile

  
Made with a medical grade rubber self healing injection port. Make huge quantities of spawn from just 1 spore syringe.

24hr pre soaked to eliminate endospores. 1x free alcohol swab included. Sterile grain spawn with injection port - 1.0 kg or 1.5 kg sterile grain spawn substrate + 1 alcohol swab per bag ordered. Each batch or grain is cleaned and then soaked for 24 hours to germinate foreign endospores.

The cleaned grain is then precooked for a short time to fully saturate the grain to the correct percentage. Each substrate bag is sealed first and then sterilised by computer controlled autoclave at above 15psi / 121c for 100 minutes to achieve total sterilisation. This grain is prepared using the best quality organic ingredients available to achieve fast growth and optimal colonisation. Inoculate this sterile grain with your favourite edible or novelty mushroom strain.

Our bags are the pre-sealed before sterilisation which means the substrate is never open to the air during or after the sterilisation process. Beware of poor quality'home made' injection ports made using silicone sealant.

They are prone to pull out and failure in use as the bags flex when mixing. Wear surgical gloves and a mask, clean gloved hands in 70% isopropanol alcohol. Use an alcohol wipe to sanitise your injection site for 30 seconds.

Now flame sterilise your needle red hot and then use a wipe to cool the needle. Alternatively, dispense a small quantity of culture or spore solution into something nearby, this will also cool your needle enough to inject the bag. Inject 3-5ml into the grain (depending os size of grain bag), withdraw the needle then immediately cover the injection holes with a minimum of 2 layers of 3m micropore tape. Gently agitate the grain being very careful not to tear the bag even better, use a home made glove box to carry out the above procedure. Make one easily from a clear plastic tote/storage box, cut out 2 hand holes, spray down inside and out with diluted bleach (5%bleach:99%water) or 70% isopropanol alcohol.

The more steps that you skip above, the more likely it is that you will contaminate your grain. Otherwise refrigerate for a few days if you have to. Each substrate bag is sealed first and then sterilised by autoclave at least 15psi / 121c for 100 minutes to achieve total sterilisation. We run regular quality control checks on our production by selecting sterilised bags at random. Qc bags are placed in fruiting conditions and are monitored.

We also sell many strains of gourmet edible mushroom cultures. Please have a look at our other items if you are interested. The benefits of using liquid mycelium culture include faster colonisation, more even and complete colonisation and higher reliability. You can expect liquid cultures to be up to 20% more bio-active than traditional agar to grain techniques.

This item is made of rye, wheat, gypsum and water.