Our tried and true mushroom cvg substrate is hydrated to field capacity and added to the grow bag before sterilization. Bags are pressure cooked at 17 psi for 2.5 hours to ensure contaminant-free substrate and sealed in front of a flow hood. We're excited to help you with your mycology goals please feel free to message us with any questions about our rye grain bags or general questions regarding gourmet and medicinal mushroom cultivation.