Liquid culture supercharged inoculation kit

  

Supercharged premium liquid culture inoculation kit. Ready to inoculate with spores or liquid culture of your choice. Our jars come with self healing injection port. Our broth will produce the best, thick cloudy mycelium. Expand your own liquid culture mix with supercharged mix containing peptone, dextrose and light malt extract (dlme).

This product has corn dextrose and light malt extract, with 4gm of peptone per 100gm mix. This is one of the best recipe to expand your own liquid culture as opposed to using just honey, karo or glucose syrup. This will result in extremely thick mycelium growth.

We use lab grade premium bacteriological peptone in our product. Explanation for using light malt extract. When inoculating a grain spawn, it colonises much faster since the mycelium has been predisposed to the sugar from grain (in this case barley). In the same way, when making malt extract agar plates (mea), the colonization is considerably faster as the mycelium is already used to the sugar.

These ingredients provide sugars and an assortment of basic minerals, vitamins, and salts helpful in the growth of the mushroom mycelium. Dextrose makes clear liquid culture (not cloudy), which makes it it is easier to spot contamination. Peptones and extracts are excellent natural sources of amino acids, peptides and proteins in growth media. This is mycological peptone is derived from soybean plants, commonly used in liquid culture recipe, agar recipe and also added to substrate as a supplement.

Supplementation with peptone not only stimulates the growth of mycelium but also greatly improves the quality of the projected mycelium mat. Our final broth recipe will be golden in colour, it will get clearer as the mycelium eats, once ready you will have thick cloudy mycelium in a transparent golden liquid. 3x 250ml pre sterilised jar of supercharge peptone dextrose light malt extract broth. Customised lid with fitted self healing injection port. Magnetic stir plate not included, can swirl by hand, the magnetic stir bar will break up the mycelium just fine. Our jars are ready to go, ready to inoculate with any spores or mycelium culture of your choice.