Pre-Sterilised MILLET Grains 1 Kg with self-healing injection port for mushroom growing

  
1 kg pre-sterilised millet grains with self-healing injection port for mushroom cultivation. This product is intended to be inoculated upon arrival. It's the millet, not rye which is a gold standard for mushroom mycelium growing to make your own grains. Millet stores well, and it has small kernels. For comparison, one pound of rye has 200 particles, whereas millets have 500. This provides more points of inoculation, making the mycelium spread more evenly and abundantly. When we make our grains, every step of the process is carefully thought out to ensure the highest rate of success. 100% clean reputable millet grains. Soaked for 12h in chlorine-free water. Washed 5 times to ensure throughout the cleaning. 5% gypsum added to avoid clumping. Simmered for 10 minutes to soften the grains and make it easy for the mycelium to digest the grains. Cooled down slowly over a period of 5 hours to avoid the autoclave from sucking up contaminated air when equalising the pessure.

Pre-sealed bags prior to sterilisation. Autoclave tape used to ensure the sterility process has been effective. Ensured grains have not been sprayed with fungicide. We obtain it from a reputable supplier. Our grains are intact and free of breakages after sterilisation as exposed grains can increase the chances of contamination.

Grains prepared at optimum moisture'field capacity of around 50-55%. Self-healing injection port included with every bag. Bags are assured 100% contaminant free due to their sterilization process of 3 hours at 20 psi in a commercial autoclave. Bag size: 10.16cmx 7.62cmx 45.72cm.

Filter size: 3.81cmx 3.81cm. Brand of bag- unicorn (4-t). Take the bags out of their packaging as soon as possible, and keep them at room temperature away from direct cold/heat sources. The bags need to breathe and should not be kept where there is no free airflow.

Keeping the bag in a closed environment will result in anaerobic contaminants to thrive and make the bag mouldy. We recommend that the bags are used as soon as possible to minimize the chances of contamination. Work in front of a flow hood or a still air box using a completely sterile workspace.

Sanitise the injection port, flame the needle until red, let it cool, and then inject the bag with 10cc liquid culture or a completely sterile spore syringe. Once inoculated, move the bag around without shaking. This helps the liquid to move around the bag and not settle at the bottom.

Incubate at 24 degrees celsius in a dark spot and keep all the grain bags at a distance from each other to avoid excess heat which leads to contamination. Agitate when 30-50% colonised with clean hands and do not touch the air filter or make the air filter wet. If the injection site begins to show contamination, that means either your liquid culture or spore syringe had contamination in it.

Recommended for inoculating with liquid culture 10cc, in contrast to a spore syringe, which may be full of bacterial contamination (spore syringes are best when purified with agar plates first, please adhere to this). Use the inoculated grains/fully colonised grains within 14 days to avoid loss of vigor and die back.

Always keep the grains somewhere dry. If the air filter gets wet, the contaminants can start growing on it. Our highest priority is ensuring the most pleasant experience for our customers. Simply send us your pictures regarding any issues with your bags upon arrival and prior inoculation. This item does not include mycelium. Check out our other listings to add mycelium liquid cultures to your order. We also have a listing for pre-sterlised rye grain.