Millet, unlike rye, is considered the gold standard for growing mushroom mycelium when creating spawnbecause millets have smaller kernels than rye. To illustrate the difference in size, consider this example: 0.5 kg of rye contains approximately 200 particles, while the same weight of millet has around 500 particles. The increased number of particles in millet leads to more inoculation points. Inoculation points are the locations where mycelium begins to grow and colonise the substrate.
The 500g variation is supplied as two separate 500g bags (totaling 1kg). 100% clean, reputable millet grains. 12-hour soak in chlorine-free water.Five-time washing for thorough cleaning. 5% gypsum added to prevent clumping. 10-minute simmer for easier mycelium digestion. 5-hour gradual cooldown to avoid contamination in steriliser. Autoclave tape to confirm sterility.
No fungicide-sprayed grains from a reputable supplier. Intact grains without breakages after sterilization. Optimal moisture'field capacity' (50-55%). Self-healing injection port on each bag. Size: 10.16cm x 7.62cm x 45.72cm.
Filter size: 3.81cm x 3.81cm. Brand: unicorn (4-t) with injection port. Take the bags out of their packaging as soon as possible, and keep them at room temperature away from direct cold/heat sources.The bags need to breathe and should not be kept in places where there is no free airflow. Keeping the pre-sterilised millet mushroom grain bags in a closed environment will result in anaerobic contaminants thriving and making the bag mouldy. We recommend that the bags are used as soon as possible to minimize the chances of contamination. Work in front of a flow hood or a still air box using a completely sterile workspace.
Sanitise the injection port, flame the needle until red, let it cool, and then inject the bag with 10cc liquid culture for 1kg and 5ml for 500gms grains or a completely sterile spore syringe. Once inoculated, move the bag around without shaking. This helps the liquid to move around the bag and not settle at the bottom. Incubate at 24 degrees celsius in a dark spot and keep all the grain bags at a distance from each other to avoid excess heat, which leads to contamination.Agitate when 30-50% colonised with clean hands and do not touch the air filter or make the air filter wet. If the injection site begins to show contamination, that means either your liquid culture or spore syringe had contamination in it. Recommended for inoculating with liquid culture, in contrast to a spore syringe, which may be full of bacterial contamination (spore syringes are best when purified with agar plates first; please adhere to this). Use the inoculated grains/fully colonised grains within 14 days to avoid loss of vigour and dieback. Always keep the grains somewhere dry.
If the air filter gets wet, the contaminants can start growing on it.