Pre-sterilised RYE mushroom grain bag withinj port and digital instructions Rye Grain Spawn

  
This product is intended to be inoculated upon arrival. Please scan the qr code on card that comes with the order to access complete instructions. Presterilised and ready to use. When we make our grains, every step of the process has been carefully thought out to ensure the highest rate of success. The 500g variation is supplied as two separate 500g bags (totaling 1kg). 100% clean and reputable grains. Soaked for 12h in chlorine-free water.

Washed 5 times to ensure throughout the cleaning. 5% gypsum is added to avoid clumping. Simmered for 10 minutes to soften the grains and make it easy for the mycelium to digest the grains. Cooled down slowly for 5 hours to prevent the autoclave from sucking up contaminated air when equalising the pressure.

Pre-sealed bags prior to sterilisation. Autoclave tape used to ensure the sterility process has been effective. Ensured grains have not been sprayed with fungicide. We obtain it from a reputable supplier. Our grains are intact and free of breakages after sterilisation, as exposed grains can increase the chances of contamination. Grains prepared at optimum moisture field capacity of around 50-55%. Self-healing injection port included with every bag. These pre-sterilised rye mushroom grain bags are assured 100% contaminant-free due to their sterilisation process of 3 hours at 20 psi in a commercial autoclave. Take the bags out of their packaging as soon as possible, and keep them at room temperature away from direct cold/heat sources. The bags need to breathe and should not be kept in places where there is no free airflow. Keeping the bag in a closed environment will result in anaerobic contaminants thriving and making the bag mouldy. We recommend that the bags are used as soon as possible to minimize the chances of contamination. Work in front of a flow hood or a still air box using a completely sterile workspace. Sanitise the injection port, flame the needle until red, let it cool, and then inject the bag with 10cc liquid culture for 1kg and 5ml for 500gms grains or a completely sterile spore syringe. Once inoculated, move the bag around without shaking. This helps the liquid to move around the bag and not settle at the bottom.

Incubate at 24 degrees celsius in a dark spot and keep all the grain bags at a distance from each other to avoid excess heat, which leads to contamination. Agitate when 30-50% colonised with clean hands and do not touch the air filter or make the air filter wet. If the injection site begins to show contamination, that means either your liquid culture or spore syringe had contamination in it. Recommended for inoculating with liquid culture, in contrast to a spore syringe, which may be full of bacterial contamination (spore syringes are best when purified with agar plates first; please adhere to this). Use the inoculated grains/fully colonised grains within 14 days to avoid loss of vigour and dieback.

Always keep the grains somewhere dry. If the air filter gets wet, the contaminants can start growing on it.