Use an injection port to inject spores or liquid culture anywhere from 5 ml to 10 ml. Every mycelium colonizes at around 22-24 c and each mycelium has its fruiting temperature.
Both rye and millet will support the growth of every fungus that people normally grow. (just leave a note for us to send a mix of rye and millet). Rye is more nutritious whereas millet has more inoculation points to reduce contamination risk. If you want to know more which grain is the best, please view this article. Do not leave your jars inside a cupboard for colonization for storage before inoculation is fine.
They need to sit somewhere with fresh airflow but away from direct sunlight or direct airflow while mycelium is colonizing the grains. Do not store unused jars inside the fridge. Only fully colonized jars can go inside the fridge to slow down the growth of the mycelium.
Use within 2 weeks of dispatch. Please shake the jar thoroughly to redistribute moisture.
The jars are checked for leakage and monitored for sterilization, most of the time contamination happens due to contaminated liquid culture or spore syringe. It is best to drop a bit of spore/liquid culture in an agar plate before inoculating these jars to check if the contamination happened due to faulty jars or liquid culture.
Please carefully inspect the jars for breakage, including any cracks that may only be visible on careful inspection. We cannot replace it unless you bring the jar back to us(sydney residents). 1be ready to move to the next step once the grain jars are fully colonized, do not leave your grains inside the jar for long otherwise, they will harden and become a rigid rock and will be impossible to take out.
2when reusing the jar and lid, please do not fill your jar more than 60-70%. Both jars and lids are autoclavable. 3please do not open the jar lid as the content won't remain sterile anymore. Only open them after full colonization. 4if your jar is contaminated, do not throw the jar or the lid, empty the contents outside in the bin or the garden and wash the jar and the lid with either hot water and soap or bleach. The lid, injection port, and air filter are reusable, you can also replace the injection port and air filter if you want after every use. 5while shaking the jar once or twice can speed up the growth, do not overdo it. It is recommended to shake once at 0% and again at 90%. Shaking at 90% is an absolute must to ensure even colonization. 6please do not forget to label your jar with species of inoculation and the date and type of grain as the grain type may not be visible after full colonization unless it is broken up.7apart from using these jars for grains, they can also be used to make liquid culture. Do not bang the glass jar on your opposite hand to break the clumps and the glass may break and pierce your arms, instead hot on a soft surface like a couch, or wrapped-up towel.
This item is made of glass, plastic, air filter, injection port, marble and magnetic stirrer bar.